The Histology Laboratory

The histology laboratory at the Orthopaedic Spinal Institute processes a wide variety of tissue samples including, but not limited to: vertebral motion segments, intervertebral cage devices, bone defects, and bone implants.

Tissue Samples


Undecalcified Histology

Specimens are embedded in Poly methyl methacrylate (PMMA) and are grinded down to approximately 100um using the EXAKT Microgrinding System. The resulting histologic slides depict a highly readable and accurate assessment of trabecular bone area at an implant interface, wear debris, signs of inflammatory giant cell reactions, and degenerative changes or autolysis.


Decalcified Histology

Bone specimens are decalcified using either a 10% EDTA solution or 5% formic acid solution depending on the experimental end point. They are then processed through a series of solutions and embedded in paraffin, using a Leica SM2010R Sliding Microtome with a specialized cassette holder to allow for super mega cassette sized samples (25x30x4mm). Specimens are typically prepared at 5 microns, but may be cut to other specifications.

Stains:

Immunohistochemistry

We can perform a variety of immunohistochemical assays to identify cytokine and/or macrophage activity. Please contact us directly for a consultation.